| 產品名稱 |
La1 [anti-La/SSB] |
| 商品貨號 |
B164953 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody is specific for bovine La/SSB, but can be used in an indirect ELISA to detect anti La/SSB antibodies in human sera. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Animals were immunized with purified homogeneous bovine La/SSB. Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against bovine La/SSB (autoimmune RNA protein) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against bovine La/SSB (autoimmune RNA protein) |
| Comments |
Animals were immunized with purified homogeneous bovine La/SSB. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody is specific for bovine La/SSB, but can be used in an indirect ELISA to detect anti La/SSB antibodies in human sera. Some cross-reactivity with Ro/SSB is seen in this assay. |
| Complete Growth Medium |
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%;
fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
fetal bovine serum, 90%; DMSO, 10% |
| Isotype |
IgG |
| Name of Depositor |
University of Oklahoma |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Harley JB. Monoclonal antibodies against autoimmune RNA proteins. US Patent 4,784,942 dated Nov 15 1988
|
