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Cultures can be maintained by the addition or replacement of fresh medium. Adherent cells can be dislodged by scraping and cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 10⁵ viable cells/mL. Maintain cell culture density between 10⁵ and 10⁶ cells/mL.

Temperature: 37°C
Atmosphere: 5% CO₂ 

Rudolph AK, et al. Thirteen hybridomas secreting hapten-specific immunoglobulin E from mice with Iga or Igb heavy chain haplotype. Eur. J. Immunol. 11: 527-529, 1981. PubMed: 6790293

Ishizuka T, et al. Aggregation of the FceRI on mast cells stimulates c-Jun amino-terminal kinase activity. J. Biol. Chem. 271: 12762-12766, 1996. PubMed: 8662803

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Nucleotide (GenBank) : X65775 M.musculus DNA for IgE antibody light chain (VJ).

Nucleotide (GenBank) : X65773 M.musculus DNA for IgE antibody heavy chain (VDJ).