| 產(chǎn)品名稱 |
HP6017 |
| 商品貨號 |
B164634 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
BALB/c (B cell); BALB/c (myeloma) |
| Applications |
The antibody produced by this cell line was selected as a reference reagent of the WHO Collaborating Center at the Centers for Disease Control for Human Immunoglobulin Subclasses. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Animals were immunized with purified human immunoglobulin.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human IgG (Fc) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human IgG (Fc) |
| Comments |
Antibody reacts with human IgG (all four IgG subclasses) and cross-reacts with IgG from rabbit, goat, sheep and horse.
The antibody does not react with human Fab or F(ab')2 nor does it react with human IgA, IgE or IgM.
Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, (ATCC 30-2002). To make the complete growth medium, add the following components to the base medium:
- 10 mM HEPES
- 0.05 mM 2-Mercaptoethanol
- Fetal Bovine Serum (FBS; ATCC 30-2020) to a final concentration of 10%
|
| Subculturing |
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Isotype |
IgG2a |
| Name of Depositor |
CB Reimer |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Reimer CB, et al. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma 3: 263-275, 1984. PubMed: 6209201
Jefferis R, et al. Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: Results of an IUIS/WHO collaborative study. Immunol. Lett. 10: 223-252, 1985. PubMed: 3899923
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