| 產(chǎn)品名稱 |
HCT-8 [HRT-18] |
| 商品貨號(hào) |
B164582 |
| Organism |
Homo sapiens, human |
| Tissue |
colon |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
ileocecal colorectal adenocarcinoma |
| Age |
67 years adult |
| Gender |
male |
| Storage Conditions |
liquid nitrogen vapor phase |
| Clinical Data |
male |
| Genes Expressed |
carcinoembryonic antigen (CEA) 0.5 ng/106 cells/10 days; alkaline phosphatase. The cells are positive for keratin by immunoperoxidase staining. |
| Cellular Products |
carcinoembryonic antigen (CEA) 0.5 ng/10 exp6 cells/10 days; alkaline phosphatase; keratin |
| Tumorigenic |
Yes |
| Effects |
Yes, in nude mice
Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells
|
| Comments |
The HCT-8 line is identical to the HRT-18 cell line.
The cells are positive for keratin by immunoperoxidase staining.
|
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- To remove trypsin-EDTA solution, transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to10 minutes.
- Discard supernatant and resuspend cells in fresh growth medium.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X,Y CSF1PO: 12 D13S317: 8,11 D16S539: 12,13 D5S818: 13 D7S820: 10,12,11.3 THO1: 7,9.3 TPOX: 8,11 vWA: 18,19 |
| Isoenzymes |
AK-1, 1 ES-D, 1-2 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1 PGM3, 1 |
| Name of Depositor |
A Sobrero |
| Deposited As |
Homo sapiens |
| References |
Tompkins WA, et al. Cultural and antigenic properties of newly established cell strains derived from adenocarcinomas of the human colon and rectum. J. Natl. Cancer Inst. 52: 1101-1110, 1974. PubMed: 4826581
Nelson-Rees WA, et al. Distinctive banded marker chromosomes of human tumor cell lines. Int. J. Cancer 16: 74-82, 1975. PubMed: 1058173
White LJ, et al. Attachment and entry of recombinant norwalk virus capsids to cultured human and animal cell lines. J. Virol. 70: 6589-6597, 1996. PubMed: 8794293
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