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HCC1739 BL

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編號:B164564

品牌:Mingzhoubio

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產品名稱	HCC1739 BL
商品貨號	B164564
Organism	Homo sapiens, human
Tissue	peripheral blood
Cell Type	B lymphocyte; Epstein-Barr virus (EBV) transforme
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension, The cells grow as floating aggregates of large cell clusters.
Biosafety Level	2 Cells contain Herpesvirus Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	Carcinoma
Age	51 years
Gender	female
Storage Conditions	liquid nitrogen vapor phase
Derivation	HCC1739 BL is a B lymphoblastoid cell line initiated by Epstein-Barr virus (EBV) transformation of peripheral blood lymphocytes obtained from a 51 year old White female with primary ductal carcinoma of the breast.
Clinical Data	HCC1739 BL is a B lymphoblastoid cell line initiated by Epstein-Barr virus (EBV) transformation of peripheral blood lymphocytes obtained from a 51 year old White female with primary ductal carcinoma of the breast. female
Comments	HCC1739 BL is a B lymphoblastoid cell line initiated by Epstein-Barr virus (EBV) transformation of peripheral blood lymphocytes obtained from a 51 year old White female with primary ductal carcinoma of the breast.
Complete Growth Medium	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing	Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2 x 10⁵ viable cells/mL. Maintain cultures at cell concentrations between 2 x 10⁵and 2 x 10⁶ viable cells/mL. Medium Renewal: Add fresh medium (depending on cell density) every 2 to 3 days
Cryopreservation	Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Name of Depositor	AF Gazdar, AK Virmani
References	Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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