| 產品名稱 |
E5BB3IIA2 |
| 商品貨號 |
B164388 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody induces histamine release from human basophils. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Animals were immunized with purified IgE myeloma protein. Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human IgE |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human IgE |
| Comments |
Animals were immunized with purified IgE myeloma protein. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody induces histamine release from human basophils. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Culture Medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine
adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, and 1.0 mM
sodium pyruvate, 90%; fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG2a; kappa light chain |
| Name of Depositor |
RP Siraganian |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Hook WA, et al. Detection of different antigenic sites on human IgE using monoclonal antibodies. Fed. Proc. 42: 713, 1983.
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