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CL2

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編號:B164265

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產品名稱	CL2
商品貨號	B164265
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type	hybridoma: B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	The antibody reacts with human E-selectin (endothelial leukocyte adhesion molecule 1 (ELAM-1)); CD62E. The antibody cross-reacts with canine L-selectin.
Storage Conditions	liquid nitrogen vapor phase
Derivation	Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
Genes Expressed	immunoglobulin; monoclonal antibody; against human E-selectin (endothelial leukocyte adhesion molecule 1 (ELAM-1)); CD62E.
Cellular Products	immunoglobulin; monoclonal antibody; against human E-selectin (endothelial leukocyte adhesion molecule 1 (ELAM-1)); CD62E.
Comments	Animals were immunized with human umbilical vein endothelial cells (HUVEC) that had been stimulated with recombinant interleukin-1 (IL-1). Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The antibody reacts with human E-selectin (endothelial leukocyte adhesion molecule 1 (ELAM-1)); CD62E. The antibody cross-reacts with canine L-selectin.
Complete Growth Medium	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing	Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10⁵viable cells/mL. Maintain cell density between 5 x 10⁴ and 1 x 10⁶ viable cells/mL. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation	Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Isotype	IgG2a; kappa light chain
Name of Depositor	C Smith
Deposited As	mouse (B cell); mouse (myeloma)
References	Kishimoto TK, et al. Antibodies against human neutrophil LECAM-1 (LAM-1/Leu-8/DREG-56 antigen) and endothelial cell ELAM-1 inhibit a common CD18-independent adhesion pathway in vitro. Blood 78: 805-811, 1991. PubMed: 1713515 Abbassi O, et al. Canine neutrophil margination mediated by lectin adhesion molecule-1 in vitro. J. Immunol. 147: 2107-2115, 1991. PubMed: 1717549 Abbassi O, et al. E-selectin supports neutrophil rolling in vitro under conditions of flow. J. Clin. Invest. 92: 2719-2730, 1993. PubMed: 7504692 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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