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貨號：B245025
名稱：Acanthamoeba healyi Moura et al.

貨號：B245024
名稱：Saccharomyces cerevisiae Meyen ex E.C. Hansen

貨號：B245023
名稱：Saccharomyces cerevisiae Meyen ex E.C. Hansen

貨號：B245022
名稱：Eutypella vitis (Schweinitz) Cooke emend. Glawe

貨號：B245021
名稱：Streptomyces scabiei (Thaxter) Lambert and Loria

貨號：B245020
名稱：Graphium cuneiferum (Berkeley et Broome) Mason et Ellis

貨號：B245019
名稱：HFBCI28

貨號：B245018
名稱：Septoria passiflorae Sydow, anamorph

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名稱：Dictyostelium purpureum Olive

貨號：B245016
名稱：Y71

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C180 [Clone 180]

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編號:B164075

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產品名稱	C180 [Clone 180]
商品貨號	B164075
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type	hybridoma: B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Strain	Strain:? BALB/c (B cell); BALB/c (myeloma)
Applications	The antibody reacts preferentially with the a and b isoforms of KTI and binds equally well to KTI - trypsin complexes. It reacts with native as opposed to denatured KTI.
Disclosure	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation	Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed	immunoglobulin; monoclonal antibody; against Kunitz soybean trypsin inhibitor
Cellular Products	immunoglobulin; monoclonal antibody; against Kunitz soybean trypsin inhibitor
Tumorigenic	Yes
Effects	Yes, forms ascites in BALB/c mice
Comments	Animals were immunized with Kunitz soybean trypsin inhibitor (KTI). Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts preferentially with the a and b isoforms of KTI and binds equally well to KTI - trypsin complexes. It reacts with native as opposed to denatured KTI.
Complete Growth Medium	Culture medium: RPMI 1640 with 2 mM L-glutamine, 1 mM sodium pyruvate and 10 mM HEPES, 85%; horse serum, 15%.
Subculturing	Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation	culture medium 95%; DMSO, 5%
Culture Conditions	Temperature: 37.0°C
Isotype	IgG1; kappa light chain
Name of Depositor	The United States of America
U.S. Patent Number	4,959,310
References	Brandon DL, et al. Monoclonal antibodies to soybean Kunitz trypsin inhibitor and immunoassay methods. US Patent 4,959,310 dated Sep 25 1990 Allison AC, et al. Monoclonal antibodies for interleukin-1.beta. US Patent 4,935,343 dated Jun 19 1990 . . J. Food Sci. 53: 97-101, 1988. J. Agric. Food Chem. 36: 1336-1341, 1988. . . Crop Sci. 32: 1502-1505, 1992. J. Agric. Food Chem. 39: 327-335, 1991. . Nutritional and toxicologial consequences of food processing. New York: Plenum Press; 1991. . Protease inhibitors as cancer chemopreventive agents. New York: Plenum Press; 1993.

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