| 產(chǎn)品名稱(chēng) |
C1498 |
| 商品貨號(hào) |
B164071 |
| Organism |
Mus musculus, mouse |
| Cell Type |
lymphoblast |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
acute myeloid leukemia |
| Gender |
female |
| Strain |
C57BL/6J |
| Applications |
Tested and found negative for ectromelia virus (mousepox). They are resistant to 0.1 mM cortisol. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Clinical Data |
female |
| Antigen Expression |
Antigen expression: The cells do not express B7-1. |
| Comments |
They are resistant to 0.1 mM cortisol. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can beestablished by centrifugation with subsequent resuspension in fresh medium at 1 to 3 x 105 viable cells/mL. A saturation density of 1 to 2 X 106 is obtainable.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation |
Freeze medium: Complete growth medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37°C |
| Name of Depositor |
P Ralph |
| Deposited As |
Mus musculus |
| References |
Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304
Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714
Boyer MW, et al. Thr role of B7 costimulation by murine acute myeloid leukemia in the generation and function of a CD8+ T-cell line with potent in vivo graft-versus-leukemia properties. Blood 89: 3477-3485, 1997. PubMed: 9129056
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