| 產(chǎn)品名稱(chēng) |
BE(2)-C |
| 商品貨號(hào) |
B164018 |
| Organism |
Homo sapiens, human |
| Tissue |
brain; derived from metastatic site: bone marrow |
| Cell Type |
neuroblast, Neuroblastoma |
| Product Format |
frozen |
| Morphology |
neuroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
neuroblastoma |
| Age |
2 years |
| Gender |
male |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
BE(2)-C is a clone of the SK-N-BE(2) neuroblastoma cell line (see ATCC CRL-2271) that was established in November of 1972 from a bone marrow biopsy taken from child with disseminated neuroblastoma after repeated courses of chemotherapy and radiotherapy. |
| Clinical Data |
BE(2)-C is a clone of the SK-N-BE(2) neuroblastoma cell line (see ATCC CRL-2271) that was established in November of 1972 from a bone marrow biopsy taken from a child with disseminated neuroblastoma after repeated courses of chemotherapy and radiotherapy. male |
| Comments |
BE(2)-C cells have a reported saturation density of greater than 5 X 105 cells/cm2.
The cells grow as clusters of flattened neuroblastic cells with occasional fine cell processes (neurites).
Unlike the parent line, they generally do not detach and float. |
| Complete Growth Medium |
The base medium for this cell line is a 1:1 mixture of ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C
Subculture Ratio: 1:4 to 1:6
Medium Renewal: Every 2 to 3 days.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
|
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 9,11 D5S818: 12 D7S820: 9,10 THO1: 6 TPOX: 11 vWA: 18 |
| Population Doubling Time |
18 hrs |
| Name of Depositor |
JL Biedler |
| Deposited As |
Homo sapiens |
| Year of Origin |
November, 1972 |
