| 產(chǎn)品名稱 |
A-704 |
| 商品貨號 |
B163890 |
| Organism |
Homo sapiens, human |
| Tissue |
kidney |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
adenocarcinoma |
| Age |
78 years |
| Gender |
male |
| Applications |
This cell line is a suitable transfection host. ref |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
(P59) diploid to hyperdiploid, hypertriploid to hypertetraploid with abnormalities including breaks, dicentrics and endoreduplication |
| Derivation |
The A-704 line was derived by D.J. Giard as indicated in the description for ATCC HTB-41. |
| Clinical Data |
male |
| Tumorigenic |
No |
| Effects |
No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| STR Profile |
Amelogenin: X,Y CSF1PO: 7,8 D13S317: 8 D16S539: 12,13 D5S818: 10,11 D7S820: 10 THO1: 7,9 TPOX: 11 vWA: 14,18 |
| Isoenzymes |
AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1 PGM3, 1-2 |
| Name of Depositor |
DJ Giard |
| Deposited As |
Homo sapiens |
| References |
Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758
Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047
|
| Cross References |
Nucleotide (GenBank) :
U02327
Human clone ndf19 neu differentiation factor mRNA, partial cds.
Nucleotide (GenBank) :
U02330
Human clone ndf33 neu differentiation factor mRNA, partial cds.
Nucleotide (GenBank) :
U02326
Human clone ndf43 neu differentiation factor mRNA, complete cds.
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