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4H1-A7

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編號:B163771

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產品名稱	4H1-A7
商品貨號	B163771
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type	hybridoma: B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	The antibody binds to and neutralizes CAV-2 and is type specific. Tested and found negative for ectromelia virus (mousepox).
Storage Conditions	liquid nitrogen vapor phase
Derivation	Animals were immunized with purified CAV-2 (Manhattan strain). Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed	immunoglobulin; monoclonal antibody; against canine adenovirus type 2 (CAV-2)
Cellular Products	immunoglobulin; monoclonal antibody; against canine adenovirus type 2 (CAV-2)
Tumorigenic	Yes
Effects	Yes, in BALB/c mice
Comments	Animals were immunized with purified CAV-2 (Manhattan strain). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody binds to and neutralizes CAV-2 and is type specific. Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium	Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%
Subculturing	Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 10⁵ viable cells/mL. Maintain cultures at a cell concentration between 1 x 10⁵ and 1 x 10⁶ cells/mL. Do not allow the cell concentration to exceed 1 x 10⁶ cells/mL. Medium Renewal: Two to three times weekly
Cryopreservation	Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Isotype	IgG2a
Name of Depositor	CA Whetstone
Deposited As	mouse (B cell); mouse (myeloma)
References	Whetstone CA. Monoclonal antibodies to canine adenoviruses 1 and 2 that are type- specific by virus neutralization and immunofluorescence. Vet. Microbiol. 16: 1-8, 1988. PubMed: 3281371 Whetstone CA. Should the criteria for species distinction in adenoviruses be reconsidered? Evidence from canine adenoviruses 1 and 2. Intervirology 23: 116-120, 1985. PubMed: 3884549 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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