| 產品名稱 |
1H10-6 |
| 商品貨號 |
B163625 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Dengue |
| Strain |
Strain:? BALB/c (B cell); BALB/c (myeloma) |
| Applications |
Animals were immunized with dengue virus type 4 antigen prepared in mouse brains (the H241 strain was used). The antibody is type specific. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with myeloma P3X63Ag8 cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against dengue virus type 4 (denguevirus-4) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against dengue virus type 4 (denguevirus-4) |
| Tumorigenic |
Yes |
| Effects |
Yes, in BALB/c mice |
| Comments |
Animals were immunized with dengue virus type 4 antigen prepared in mouse brains (the H241 strain was used). The antibody is type specific. Spleen cells were fused with myeloma P3X63Ag8 cells. Antibodies should be prepared as ascites in pristane primed BALB/c mice. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Culture Medium: Modified Dulbecco's medium (see below), 90%; fetal bovine
serum, 10%.
DME w/HEPES (10mM) 100 mL
L-Glutamine (100x) 1 mL
Solution I 1 mL
Non-essential amino acids (100x) 1 mL
NCTC 135 10 mL
Fetal Bovine Serum 12 mL
Solution I (100x)
1. 1320 mg oxalacetic acid (100 mM, MW 132).
2. 80 mg crystalline bovine insulin (20 units/mL, 25 units/mg).
Add 1 and 2, stir at 37°C. Add Na pyruvate 550 mg (50 mM, FW 110). Bring up to
100 mL with distilled water. Stir at 37°C until solution dissolves. Filter,
aliquot and store frozen. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
MK Gentry |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Henchal EA, et al. Dengue virus-specific and Flavivirus group determinants identified with monoclonal antibodies by indirect immunofluorescence. Am. J. Trop. Med. Hyg. 31: 830-836, 1982. PubMed: 6285749
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