| 產(chǎn)品名稱 | UACC-1179 |
|---|---|
| 商品貨號 | B162142 |
| Organism | Homo sapiens, human |
| Tissue | breast Derived from metastatic site: pleural fluid |
| Cell Type | epithelial-like |
| Product Format | frozen |
| Morphology | epithelial-like |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | adenocarcinoma |
| Age | 61 years old |
| Gender | female |
| Applications | Theses cells have been used in studies of the p53 mutation, as well as the regulation of p53 on DSC3 and MAPSIN.
|
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | range=32-75; modal number=34-35; 9pHSR |
| Images |  |
| Derivation | UACC-1179 was derived from a 61 year-old female with breast cancer. The patient underwent chemotherapy |
| Receptor Expression | neu (ErbB2; HER2), expressed epidermal growth factor receptor (EGFR), not expressed estrogen receptor (ER), not expressed progesterone receptor (PR), not expressed |
| Comments | UACC-1179 is negative for tumor suppressor genes MASPIN and the cell adhesion molecule DSC3.
Inhibition of DNA methyltransferase could induce transcriptional reactivation of both DSC3 and MASPIN of this cell line.
The p53R213X mutation has been reported in this cell line.
The cells have been used in studies of p53 mutation, as well as the relegation of p53 on DSC3 and MAPSIN. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium:
|
| Subculturing | These cells are cultured on collagen I coated vessels. Add 5 µg per cm2 collagen I (BD Biosciences, Cat. No.354236 or equivalent) to culture vessels and incubate at room temperature for 1 hour. Remove collagen solution and rinse vessels 3 times with a balanced salt solution. Vessels may be used immediately or air dried and stored at 2-8°C for up to one week under sterile conditions. Alternatively, commercially available pre-coated Collagen I vessels, such as BD BioCoat Cellware (BD Biosciences, Cat. No. 356524 for 75 cm2 flask) or equivalent, may be used.
Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of solutions for culture vessels of other sizes.
Subcultivation ratio: A subcultivation ratio of 1:2 is recommended.
Medium renewal: every 2 to 3 days Note: This cell line grows slowly. |
| Cryopreservation | Freeze medium: complete growth medium, 80%; FBS, 10%; DMSO, 10% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37.0°C
Atmosphere: air, 100% |
| STR Profile | D5S818: 12 D13S317: 12 D7S820: 8, 11 D16S539: 10, 13 vWA: 16 TH01: 6 Amelogenin: X TPOX: 8 CSF1PO: 10 |
| Name of Depositor | K Brown |
| References | Rice JC, et al. Aberrant methylation of the BRCA1 CpG island promoter is associated with decreased BRCA1 mRNA in sporadic breast cancer cells. Oncogene 17(14): 1807-1812, 1998. PubMed: 9778046 Domann FE, et al. Epigenetic silencing of maspin gene expression in human breast cancers. Int. J. Cancer. 85(6): 805-810, 2000 PubMed: 10709100 Oshiro MM, et al. Mutant p53 and aberrant cytosine methylation cooperate to silence gene expression. Oncogene 22(23): 3624-3634, 2003. PubMed: 12789271 Watts GS, et al. The acetyltransferase p300/CBP-associated factor is a p53 target gene in breast tumor cells. Neoplasia 6(3): 187-194, 2004. PubMed: 15153330 Futscher BW, et al. Chapter 6: Epigenetic Dysregulation of Maspin (SerpinB5) in Cancer Invasion and Metastasis. DNA Methylation, Epigenetics and Metastasis. Cancer Metastasis - Biology and Treatment, 7: 133-155, 2005. Oshiro MM, et al. Epigenetic silencing of DSC3 is a common event in human breast cancer. Breast Cancer Res. 7(5): R669-R680, 2005. PubMed: 16168112 Futscher BW, et al. Chapter 7: Epigenetic Regulation of Genes That Affect Tumor Cell Adhesion. Cell Adhesion and Cytoskeletal Molecules in Metastasis. Cancer Metastasis - Biology and Treatment, 9: 123-140, 2006. Wozniak RJ, et al. 5-Aza-2'-deoxycytidine-mediated reductions in G9A histone methyltransferase and histone H3 K9 di-methylation levels are linked to tumor suppressor gene reactivation. Oncogene 26(1): 77-90, 2007. PubMed: 16799634 Robey IF, et al. Regulation of the warburg effect in early-passage breast cancer cells. Neoplasia 10(8): 745-756, 2008. PubMed: 18670636 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
