Insert contains the following sites separated by (bp): EcoRI- 199- EcoRV- 19- KpnI- 87- BglII- 70- KpnI- 23- BamHI- 61- EcoRI. Restriction digests of the clone give the following sizes (kb): HindIII--3.1; SalI--3.1; BamHI--3.1; EcoRI--2.5, 0.58; BglII--3.1. Insert is a cDNA clone of human cytochrome c oxidase subunit VIb. The amino acid sequence shows 85% homology with bovine heart cytochrome c oxidase subunit VIb. |
