人妻少妇偷人精品无码丨色婷婷av久久久久久久丨欧美xxxx做受性欧美88丨欧洲女人牲交视频免费丨亚洲精品久久久av无码专区

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > pDMS197
▍最近瀏覽歷史
▍聯系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮海區莊市街道興莊路9號
  • 創e慧谷42號樓B幢401室
pDMS197
pDMS197
規格:
貨期:
編號:B161742
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pDMS197
商品貨號 B161742
Designations pDMS197
Depositors DM Schifferli
Biosafety Level 1
Host Escherichia coli SM10 lambda pir
Vector Information
Size (kb): 6.50
Type of vector: plasmid
Cloning sites: BamHI XbaI KpnI SacI SmaI
Polylinker sites: BamHI HindIII XbaI KpnI SphI EcoRV SacI SmaI EcoRI
Vector: pDMS197 (plasmid)
Construction: pRE107, tetR (pBR322)
Host range: broad host range; Escherichia coli
Features (with orientation and position when available):
MCS: BamHI...EcoRI, ->
marker(s): sacB1, ->
restriction site: EcoRI HindIII, ->
replicon: conditional R6K ori
replicon: oriT
marker(s): tetR, ->
Applications Counterselection levansucrase
Suicide vector
Vector permitting allelic exchange of cloned inserts
Comments Restriction digests of the clone give the following sizes (kb): XbaI-- 6.55; KpnI-- 6.55; HindIII-- 2.9, 2.2, 1.4.

The sacB1 allele is a variant of sacB with certain restriction sites removed by site directed mutagenesis. Expression of sacB confers sensitivity to sucrose.

The conditional R6K origin of replication requires that the pi protein be expressed in trans for plasmid maintenance.

One of four allelic exchange suicide vectors (ATCC 87691 - 87694) that provide both selection for chromosomal integration (ampR, cmlR, kanR or tetR) and counterselection for loss of vector DNA and the wild type allele.

Construct is suicide plasmid in any host not expressing pir.

Cloned inserts may be integrated into the host chromosome (a single recombination event) following electroporation and appropriate antibiotic selection.

Negative selection for sucrose sensitivity (sacB) selects for a second recombination event resulting in loss of vector DNA.
Media ATCC® Medium 1631: LB medium (ATCC medium 1065) with 10 mcg/ml tetracycline
Growth Conditions
Temperature: 37°C
References

Edwards RA, et al. Improved allelic exchange vectors and their use to analyze 987P fimbria gene expression. Gene 207: 149-157, 1998. PubMed: 9511756

Donnenberg MS, Kaper JB. Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector. Infect. Immun. 59: 4310-4317, 1991. PubMed: 1937792

Blomfield IC, et al. Allelic exchange in Escherichia coli using the Bacillus subtilis sacB gene and a temperature-sensitive pSC101 replicon. Mol. Microbiol. 5: 1447-1457, 1991. PubMed: 1686293

Kolter R, et al. Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K. Cell 15: 1199-1208, 1978. PubMed: 728998

McIver KS, et al. The elastase propeptide functions as an intramolecular chaperone required for elastase activity and secretion in Pseudomonas aeruginosa. Mol. Microbiol. 18: 877-889, 1995. PubMed: 8825092

Shipped frozen
梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
主站蜘蛛池模板： 亚洲精品中文字幕一区二区三区| 女人被爽到呻吟gif动态图视看| 欧洲熟妇色xxxx欧美老妇软件| 国产露脸150部国语对白| 国产男女爽爽爽免费视频| 国模裸体无码xxxx视频| 久久视频这里有精品33| 亚洲视频日本有码中文| 精品国产福利在线视频| 波多野结衣超清无码专区| 92精品成人国产在线观看| 中文字幕无码无码专区| 加勒比久久综合网天天| 国产免国产免费| 国产爆乳无码视频在线观看| 免费国产成人午夜福利电影| 2021精品国产自在现线看| 国产一区二区三精品久久久无广告| 成人午夜福利视频镇东影视| 国产网红主播无码精品| 麻豆国产在线精品国偷产拍| 日韩免费一区二区三区高清| 亚洲熟妇av一区| www插插插无码免费视频网站 | 国产国拍精品av在线观看| 92国产精品午夜福利无毒不卡| 色久悠悠婷婷综合在线亚洲 | 羞国产在线拍揄自揄视频| 亚洲国产av美女网站| 乱码午夜-极品国产内射| 日韩精品无码人妻一区二区三区| 免费午夜理论不卡| 中文字幕亚洲欧美在线不卡| 性色av免费观看| 久久久久青草线综合超碰| 女人爽到高潮视频免费直播| 影音先锋亚洲成aⅴ人在| 精品人妻无码专区中文字幕| 老司机在线精品视频网站| 成人亚洲欧美激情在线电影| 國产一二三内射在线看片 |